FIG. 2.

Immunoglobulin responses to the HC₅₀ domain of BoNT/A when it was coadministered with an adjuvant. (a) The polypeptide was administered to mice (10 mice per group) as an i.n. vaccine with chitosan (▪) or with vitamin E (•), and the magnitudes of the IgG responses were determined. (b) The polypeptide was administered to mice (10 mice per group) as an i.n. vaccine with chitosan (⧫) or with vitamin E (▴), and the magnitudes of the IgA responses were determined. The adjuvants enhanced the responses to both immunoglobulins, but the effect with IgA was greater. This was probably a reflection of the fact that the response to IgA in the absence of adjuvant (see Fig. 1) was lower.