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Immunoglobulin responses to the HC50 domain of BoNT/A administered as an injection (i.m.) vaccine. The polypeptide was given to mice (10 mice per group) in the absence (open symbols) or in the presence (solid symbols) of an adjuvant (alum). (a) Magnitudes of IgG responses. (b) Magnitudes of IgA responses. The adjuvant enhanced the responses to both classes of immunoglobulin, and the magnitude of the enhancement was substantial for IgA.
