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Transcytosis of BoNT/A HC50 (gel A), BoNT/B HC50 (gel B), and BoNT/E HC50 (gel E). Equimolar concentrations of the three polypeptides (1 × 10−8 M) or the corresponding parent toxins were added to the upper wells of T-84 monolayers in a Transwell apparatus, and the accumulation in the lower wells was assayed by Western blotting. Lane 1, holotoxin added; lane 2, HC50 domains added. Note that the levels of transcytosis for each holotoxin and its HC50 domain were comparable.
