FIG. 6.

Individual and combined rates of transcytosis of BoNT/A HC₅₀ (gel A), BoNT/B HC₅₀ (gel B), and BoNT/E HC₅₀ (gel E). Equimolar concentrations of the three polypeptides (1 × 10⁻⁸ M) were added to the apical surfaces of T-84 monolayers, and the accumulation of the polypeptides on the basal side of the cells was assayed by Western blotting. Lane 1, HC₅₀ domain added alone; lane 2, three HC₅₀ domains added together. The results indicate that simultaneous addition of HC₅₀ domains did not inhibit or reduce the extent of transcytosis of any of the domains.