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Immunoglobulin responses to mixed-mode administration of a trivalent vaccine. The HC50 domains of three serotypes were administered as described in the text, and the magnitudes of the IgG responses (a) and IgA responses (b) were determined. The results clearly show that all three antigens evoked substantial responses. ▴, BoNT/A; ▪, BoNT/B; •, BoNT/E.
