FIG. 1.

SERPA of B. anthracis secreted proteins: comparison of Coomassie blue-stained 2-DE (gels 1, 3, and 5) with the corresponding Western blots (gels 2, 4, and 6). (Gels 1 and 2) FAG medium secretome of the Vollum strain. (Gels 3 and 4) BHI medium secretome of the Vollum strain. (Gels 5 and 6) FAG medium secretome of the Δ14185 strain. Blots were probed with the sera indicated (see Materials and Methods for a description of the sera). See Table 1 for the identities of the marked protein spots and a complete list of immunogenic proteins. The protein spot numbering is the numbering on the proteomic maps reported previously (16). Also see Fig. S1 in the supplemental material for an extended overview. MW, molecular weight.