TABLE 1.

Immunogenic B. anthracis proteins identified by SERPA

Accession no.a	Proteinb	Domain(s)c	Expression under high-CO2 conditionsd	Membranal fractione	2-DE spotf	Confirmed by IPg	Virulence relatedh
pXO1-90	Unknown	S, SLH	+		51	+
pXO1-107	LF	S	+		52	+	+
pXO1-110	PA	S	+		6	+	+
pXO1-122	EF	S	+		60	+	+
pXO1-130	SBP-ABC [Zn] (AdcA)	S	+		73	+
BA0108	Elongation factor (EF-Tu)	S		+
BA0165	Prolyloligopeptidase	S			36		+
BA0228	ATP-ABC (YdiF)		+		23
BA0267	Heat shock protein (GroEL)		+	+	4	+	+
BA0307	Unknown lipoprotein (YerH)	S	+		61
BA0309	δ-1-Pyrroline-5-carboxylase (RocA)			+		+
BA0345	Hydroperoxide reductase (AhpC)			+	67	+	+
BA0656	SBP-ABC [oligopeptide]	S	+		8	+	+
BA0796	Unknown	S, SH3, 3D	+		56	+
BA0799	Unknown	S, HlyD	+		39
BA0855	SBP-ABC [amino acid] (YckK)	S	+		30	+
BA0885	S-layer protein (Sap)	S, SLH	+	+	19	+
BA0887	S-layer protein (EA1)	S, SLH	+	+	47	+
BA0898	Alanine amidase III (CwlB)	S, SLH	+	+	50	+	+
BA0908	SBP-ABC [oligopeptide]	S			28		+
BA1075	Nuclease/phosphatase	S			54
BA1129	Unknown	S, SLH		+
BA1191	SBP-ABC [oligopeptide]	S	+		8	+	+
BA1197	SBP-ABC [oligopeptide]	S	+		20		+
BA1290	Camelysin (TasA)	S	+		21		+
BA1295	Metalloprotease (InhA1)	S	+		2	+	+
BA1436	Sulfatase (YvgJ)	S			53		+
BA1449	Peptidase M23/M37	S			42
BA1818	Alanine amidase IV	S, SLH		+		+
BA1952	NlpC/P60 endopeptidase	S, NlpC/P60, SH3	+		38	+	+
BA2283	Unknown				55
BA2346	Methylcitrate dehydratase (MmgE)			+
BA2673	Chitosanase				62
BA2944	Polysaccharide deacetylase (YjeA)	S	+		34	+	+
BA2947	Sulfatase (YflE)	S			29		+
BA3189	SBP-ABC [Mn] (MntA)	S	+	+	18	+	+
BA3338	Unknown	S, SLH		+	48
BA3346	6-Aminohexanoate dimer hydrolase	S			33
BA3367	γ-Phage receptor (GamR)	S, LPXTG	+		3	+
BA3588	Lipoprotein (VanW)	S	+		17
BA3609	Aldehyde dehydrogenase (DhaS)			+
BA3645	SBP-ABC [oligopeptide]	S		+	69		+
BA3660	Serine protease (HtrA)	S	+		11	+	+
BA3737	Alanine amidase II (CwlA)	S, SLH	+	+	49	+	+
BA4322	Nucleotidase	S	+		9		+
BA4346	2,3-Cyclic nucleotide diesterase (YfkN)	S, LPXTG	+	+	57	+	+
BA4387	Leucine dehydrogenase (LeuDH)				70
BA4539	Heat shock protein (DnaK)		+	+	1		+
BA4705	Peptidyl-prolyl isomerase (RopA)			+
BA4750	Carboxypeptidase (VanY)	S			31
BA4873	Alanine dehydrogenase (Ald-2)				71
BA5220	SBP-ABC [methionine]	S			65
BA5364	Enolase (Eno)		+		5		+
BA5427	Endopeptidase (LytE)	S, NlpC/P60	+		59	+	+
BA5470	Sulfatase (YvgJ)	S	+		10		+
BA5576	Fructose biphosphatase (GlpX)			+
BA5696	Superoxide dismutase (SodA-2)			+	64		+
BAS*5205	Collagen adhesion protein	S			27	+	+

^aSeroreactive proteins identified by serological proteome analysis are listed in ascending order of the accession number (TIGR identification tag).

^bThe designations for some of the proteins are in parentheses. The putative functional annotation of the proteins is the annotation of Ariel et al. (5, 6). The SBPs of ABC transporters are indicated by SBP-ABC, and their inferred ligand specificities (according to the TransportDB database) are indicated in brackets.

^cS, export signal; SLH, S-layer homology domain; NlpC/P60, cell wall peptidase family domain; SH3, Srk homology domain; HlyD, involved in secretion of virulent factors in gram-positive pathogens; 3D, cation binding domain involved in protein-protein interaction.

^dProteins expressed under high-CO₂ conditions, as determined previously (16), are indicated by plus signs.

^eThe table includes 20 immunogens which were identified by SERPA of B. anthracis membranal enriched fractions (6, 15); 14 of these immunogens were also identified in the bacterial secretome and therefore were assigned a spot number (16). The proteins identified only in the membranal fraction are BA0108, BA0309, BA1129, BA1818, BA2346, BA3609, BA4705, and BA5576.

^fThe numbers are the numbers assigned to the protein spots on the 2-DE maps of the secretome, as described previously (16). Some of the immunogenic protein spots are present on the gels and Western blots shown in Fig. 1.

^gThe immunogenicities of the proteins indicated by plus signs were also determined by quantitative IP of in vitro translation products with immune sera in the context of the screen described by Gat et al. (28) (group I proteins in Fig. 3).

^hOrthologs of proteins involved in virulence and pathogenicity, as described previously (16), are indicated by plus signs.