TABLE 1.

Recombinant constructs used in this study and oligonucleotide primers used to amplify the subsegments of the Acm A domain

Expression construct	Subdomain	aa	Molecular mass (kDa)b	Recombinant protein	Sequencea
pTEX5411	N2trun	151-320	20.881	rAcm21	5′-CGCGGATCCGTGACGAGCGGTGATAAAACAGCTACTG
					5′-CGGGGTACCTTAATTTTTAACTGTATGATTGAAACTTTC
pTEX5412	N2	151-346	23.667	rAcm24	5′-CGCGGATCCGTGACGAGCGGTGATAAAACAGCTACTG
					5′-CGGGGTACCTTAAATCTCTGTGTCATTGATATATTTG
pTEX5413	N2N3	151-529	44.286	rAcm44	5′-CGCGGATCCGTGACGAGCGGTGATAAAACAGCTACTG
					5′-CGGGGTACCTTATTTATTCTCATTTGTAACGACTAGC
pTEX5414	N1N2trun	29-320	34.401	rAcm34	5′-CGCGGATCCGATGCAGGCAGAGATATCAGCAGTAATG
					5′-CGGGGTACCTTAATTTTTAACTGTATGATTGAAACTTTC
pTEX5415	N1N2	29-346	37.147	rAcm37	5′-CGCGGATCCGATGCAGGCAGAGATATCAGCAGTAATG
					5′-CGGGGTACCTTAAATCTCTGTGTCATTGATATATTTG
pTEX5416	N1N2N3	29-529	57.900	rAcm58	5′-CGCGGATCCGATGCAGGCAGAGATATCAGCAGTAATG
					5′-CGGGGTACCTTATTTATTCTCATTTGTAACGACTAGC

^aThe introduced restriction sites are underlined, and stop codons are italicized.

^bMolecular masses were estimated by matrix-assisted laser desorption ionization-time of flight mass spectrometry.