TABLE 2.
Nucleotide sequences of oligonucleotide primers
| Primer | Sequence (5′→3′)a | Restriction endonuclease |
|---|---|---|
| 340 | TATACTAGTGGGTGACTAACTAGGAGGAATAAATGGCTA | SpeI |
| 481 | GCGGGTACCGTCGACTCTAGAGGATCCCCGGGTCATTA | KpnI |
| 639 | CACACCCATATGAATGAGGTTCGTAGCAGC | NdeI |
| 640 | ATGGATCCTGCCGTCTTGACCGATTTC | BamHI |
| 651 | GGGGTGGTCAGCCTAAAAAGTAAC | |
| 652 | AGTGGTGATTTGGTCGTTCTGG | |
| 659 | TATCTTTTACTGGCTCCTCC | |
| 660 | TACCTCATACCAACCGCTC | |
| 661 | CGCCTCGAGCACTAAAAGCAAAGCCTGC | XhoI |
| 662 | ATATAGATCTCCTCAATCACAAAGTCAATCTC | BglII |
| 788 | ATAGGTACCAGGGTATCAACCAATCTAATCCGC | KpnI |
| 789 | ATTCCACCTCACCACCTCTG | |
| 790 | ATACTGCAGACGATGGCGAAGTGGTTTATTG | PstI |
| 791 | GTGACTAGTGGTGGACTGTCCTTGTTGTTTTAAG | SpeI |
| 798 | TTTTGGCTCAATACCAGCATTCGG | |
| 799 | CAGGCACACTCATTCACACCATCC | |
| 802 | CGCATCAGCATCATCTTCACCGAG | |
| 803 | TACCTCATACCAACCGCTCCAGCC | |
| 814 | TATAAGATCTACGTGTTGACAATTAATCATCG | BglII |
| 815 | TATACTCGAGATTCTCAGTCCTGCTCCTCG | XhoI |
| 858 | GTGCAGCCGACTTTTCTTCC | |
| 965 | AGGTATCGCTAATGCCACTTCAAC | |
| 966 | ACTCAAGACCATAAGAGCCGTCAG | |
| 1058 | GCTTCATCAAGTTTTGCCTCC | |
| 1059 | TTTGCTTTGGTTGGGTCTG | |
| 1062 | TCTCAGATCTACAATGAATAGGTTTACAC | BglII |
| 1063 | TGTGCTCGAGGCAAGGGTTTATTGTTTTC | XhoI |
| 1213 | ACACCTCGAGCCACCATCTATGTTCAAGGCA | XhoI |
| 1214 | TCTCAGATCTGGGTTGTAAAGGTTGTGTTGG | BglII |
| 1243 | ATTACCATCACCGCAGAT | |
| 1417 | ATACCCGGGTTGACAATGGTGTGTGCCTC | |
| 1418 | ATAACTAGTTTTAGCACCGATTTTTCGC |
a
Engineered restriction endonuclease sites are underlined.