TABLE 2.
Strain | Time postinfection (h) | Fraction colocalizationa
|
|||||||
---|---|---|---|---|---|---|---|---|---|
Dextran
|
Transferrin
|
LBPA
|
LAMP-2
|
||||||
Avg | SD | Avg | SD | Avg | SD | Avg | SD | ||
Controls | |||||||||
Erdman live | 24 | 0.654 | 0.045 | 0.619 | 0.039 | 0.447 | 0.044 | 0.399 | 0.033 |
72 | 0.667 | 0.047 | 0.627 | 0.056 | 0.391 | 0.031 | 0.353 | 0.035 | |
Erdman heat killed | 24 | 0.320 | 0.045 | 0.395 | 0.053 | 0.678 | 0.051 | 0.598 | 0.027 |
72 | 0.308 | 0.059 | 0.413 | 0.041 | 0.679 | 0.038 | 0.589 | 0.049 | |
Class I mutants | |||||||||
Rv3887::Tn | 24 | 0.314 | 0.108 | 0.402 | 0.043 | 0.583 | 0.128 | 0.560 | 0.023 |
72 | 0.356 | 0.050 | 0.381 | 0.015 | 0.687 | 0.007 | 0.559 | 0.070 | |
Rv2206::Tn | 24 | 0.390 | 0.056 | 0.372 | 0.024 | 0.688 | 0.069 | 0.617 | 0.001 |
72 | 0.376 | 0.080 | 0.374 | 0.043 | 0.718 | 0.064 | 0.617 | 0.036 | |
Rv2693c::Tn | 24 | 0.440 | 0.109 | 0.401 | 0.002 | 0.664 | 0.060 | 0.605 | 0.032 |
72 | 0.365 | 0.037 | 0.344 | 0.054 | 0.614 | 0.077 | 0.570 | 0.024 | |
ΔmmpL9 | 24 | 0.464 | 0.074 | 0.330 | 0.042 | 0.638 | 0.086 | 0.519 | 0.087 |
72 | 0.404 | 0.076 | 0.353 | 0.002 | 0.614 | 0.075 | 0.587 | 0.009 | |
Class II mutants | |||||||||
pcaA::Tn | 24 | 0.637 | 0.044 | 0.589 | 0.038 | 0.387 | 0.046 | 0.429 | 0.069 |
72 | 0.338 | 0.037 | 0.442 | 0.060 | 0.580 | 0.010 | 0.571 | 0.014 | |
moeB1::Tn | 24 | 0.657 | 0.048 | 0.632 | 0.083 | 0.433 | 0.060 | 0.382 | 0.061 |
72 | 0.319 | 0.074 | 0.393 | 0.027 | 0.622 | 0.042 | 0.589 | 0.062 |
The average fraction colocalization with each marker was computed by using >200 scored phagosomes from at least three experiments.