FIG. 2.

Outer membrane subproteome for UPEC during growth in human urine. 2D gel of carbonate-insoluble membrane proteins prepared from CFT073 following culture in urine. Outer membrane fraction (150 μg protein) was isoelectrically focused on a pH 3 to 10 IPG strip, followed by 10% SDS-PAGE. The image shows the proteins identified by MS/MS (arrows). Boxed areas indicate proteins where multiple isoforms produced the same identification. The CFT073 annotated gene numbers, theoretical masses, and isoelectric points of the identified proteins are listed in Table 2.