FIG. 4.

Normalized spot volumes from multivariable 2D-DIGE scan of urine (Cy3) and LB medium (Cy5) UPEC outer membrane proteins. (A) Spot volumes for known and predicted CFT073 TonB-dependent outer membrane receptors for iron, (B) outer membrane porins, and (C) proteins showing decreased expression in urine or similar expression patterns in LB medium and urine. The 3D peaks represent the normalized pixel intensities for identical spot boundaries between the Cy3 and Cy5 scans. The boxed regions below the peaks are the corresponding protein isoforms present in the DIGE scan.