FIG. 5.

2D-DIGE expression patterns for UPEC outer membrane proteins differentially expressed in urine and LB medium. The standardized log₁₀ abundance from triplicate 2D-DIGE experiments was generated by using the internal standard included in every gel. The Cy2 internal standard signals were used to normalize the ratios of LB to urine across multiple gels. The average abundance (solid lines) and individual quantitative changes (dashed lines) between normalized Cy3 (urine) and Cy5 (LB medium) signals are shown.