FIG. 6.
qPCR for UPEC iron receptor genes during growth in urine, during growth in iron-replete urine, and during defined iron limitation. (A) qPCR results for CFT073 grown in human urine in the presence (gray bars) and absence (black bars) of 10 μM FeCl2 and (B) cultured in LB medium containing 10 mM deferoxamine mesylate, an iron-chelating agent. Data were normalized to gapA (glyceraldehyde 3-phosphate dehydrogenase) expression levels, and changes were determined by using LB medium expression levels as the calibrator. For iron-replete urine qPCR, urine without supplementation with ferrous chloride was used as the calibrator to generate n-fold change values. In panel B, the black bars represent urine transcript n-fold changes and the gray bars represent LB-deferoxamine mesylate medium transcript levels compared to LB medium transcript levels.