TABLE 1.
Oligonucleotide primers for quantitative real-time PCR
| Gene | ORF | Sequencea
|
|
|---|---|---|---|
| Forward | Reverse | ||
| gapA | c2184 | AAGTTGGTGTTGACGTTGTCGC | AGCGCCTTTAACGAACATCG |
| btuB | c4929 | TTGCGCCAACTACTGTTGTGAC | ACATGACTGGCATTTGTACCGC |
| Tsx | c0521 | ACGACAAACCGCAGTATCTTTCCG | AGCGAATGCTTCGTACTCAAGG |
| tolC | c3781 | AATGAAGCGCGCAGTCCATTAC | TTAACGCACGCCATTTCGAC |
| tonB | c1717 | TGCATTCATGGTGCTGTTGTGG | TTGTGGCGGTTCAAGATCAG |
| irgA | c5174 | TCCTGTGGAAGCAATTGAACGC | AAGTACGCCATCCCATGTTTGG |
| iha | c3610 | TGCGAATAACCACTCTGGCTTC | TAATCACAGAAACACTGGCGGC |
| NAb | c2482 | ATCGTTCGGCAAGCAACCTTTG | ATGCGGATTTGTTTACGGCCTG |
| iroN | c1250 | ATTACCAAACGTCCCACCAACG | AAACGCGTGGTAAGAGCATCAC |
| fhuA | c0185 | ACGGCCAAAGCCAGAATAAC | TTACCGTAAAGCACGGAAACCG |
| fepA | c0669 | ATTGATATTCGCGGCATGGGTC | ACGTTCAATCATTTCAGGCGGC |
| iutA | c3632 | AAAGAGCTGAAAGACGCACTGG | TGTCGGAACGTGAAGAGTTGAG |
| chuA | c4308 | AGCGTGTTGAGATTGTTCGC | AAACCACTGCTTTGTCCTTCCTGC |
| cirA | c2690 | AAAGAAGTGCCTGGCGTACAAC | AAATCATTGTGGCGGAAGACGG |
a
Primer sequences are listed from 5′ to 3′.
b
NA, no gene name available.