FIG. 4.

Western blot analysis of selected OM proteins expressed by M. catarrhalis isolates of various origins. OM vesicles were resolved on 7.5% polyacrylamide gels, transferred to PVDF membranes, and probed with murine sera against MhaC and MhaB. McaP antibodies (Ab) were also included in these experiments as loading indicators. Molecular mass markers are shown to the left in kilodaltons. This figure is a composite of separate Western blots, and therefore, O35E (lanes 1 and 10) was included as a reference strain.