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. 2007 Mar 19;75(6):2974–2980. doi: 10.1128/IAI.01915-06

FIG. 2.

FIG. 2.

Chromatographic profiles of three conjugates on a Sephacryl S-300 column (1.6 by 95 cm). The first peak around the void volume that contained both protein and carbohydrate was collected and designated dLOS-TT (A), dLOS-CRM-1 (B), or dLOS-CRM-2 (C). Unconjugated carrier proteins or AH-dLOS was eluted from fractions 53 to 58 on the same column. The void volume was around fraction 39, and the total volume was around fraction 83. Each fraction contained 2 ml eluent. The optical density at 490 (OD490) and optical density at 562 (OD562) were the optical density of the carbohydrate determined by the phenol-sulfuric acid assay (7) and the optical density of the protein determined by the micro bicinchoninic acid assay (33), respectively.