TABLE 5.
Cross-bactericidal activity of a representative rabbit serum elicited by serotype C dLOS-TT conjugate
| Straina | Serotype | Bactericidal titerb |
|---|---|---|
| 25238 | A | 1:160 |
| 26395 | A | 1:320 |
| 26394 | A | CKc |
| 3292 | B | 1:20 |
| 26397 | B | <1:5 |
| 26400 | B | 1:80 |
| 26391 | C | CK |
| 26404 | C (homologous) | 1:80 |
| O35E | A/C′d | 1:40 |
| TTA24 | B′d | 1:20 |
| 25239 | B′ | <1:5 |
| 43627 | B′ | 1:20 |
| 49143 | B′ | 1:10 |
| M1 | A/C′ | 1:80 |
| M3 | A/C′ | 1:80 |
| M4 | B′ | <1:5 |
| M6 | A/C′ | 1:80 |
| M7 | B′ | 1:10 |
| M9 | A/C′ | 1:320 |
| M10 | B′ | <1:5 |
The concentration of each strain was adjusted to 6 × 103 CFU/ml for the bactericidal assay.
See Table 4, footnote b.
CK, complement itself killed the strains without the presence of antiserum.
Assigned serotypes were based on binding activities with monoclonal antibody 8E7 against serotype A/C LOS (18) and a rabbit serum against serotype B LOS (43) as determined by whole-cell ELISA and Western blotting. Strains that bound to only monoclonal antibody 8E7 or showed strong binding activity to monoclonal antibody 8E7 were assigned to serotype A/C. In contrast, strains that bound only to the rabbit serum against serotype B or showed strong binding activity to the rabbit serum were assigned to serotype B.