PCR analysis of S. epidermidis HBmut and HB. Confirmation of allele replacement of fbe with fbe::ermB in chromosomal DNA. Lanes 1, 3, 5, and 7 are amplified products from strain HBmut. Lanes 2, 4, 6, and 8 are amplified products from strain HB. Lanes 1 and 2, primers P3L and P3R; lanes 3 and 4, primers P4L and P4R; lanes 5 and 6, primers P5L and P5R; lanes 7 and 8, primers P6L and P6R; lane M, markers.