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Expression of N. meningitidis NMB IgA1 protease wild type (WT) and mutants (1 to 12, corresponding to mutants M1 to M12). Enzyme activity was measured by quantitative ELISA with human IgA1 as a substrate, as described in Materials and Methods. V, supernatants from E. coli BL21 containing vector plasmid only (no iga gene) as negative controls showed no activity. The error bars indicate standard errors of the means.
