Figure 1.

Expression of NF-κB in stratified epithelium in vivo. Frozen tissue sections of non-sun-exposed adult human abdominal skin were immunostained with antibody to p50. Immunoperoxidase staining for (a and c) p50 and (b and d) secondary antibody alone control. Immunofluorescence staining for (e) p50 and (f) secondary antibody alone control. E, epidermis; D, dermis; SC, stratum corneum; SG, stratum granulosum; S, squamous layer; B, basal layer; BMZ, basement membrane zone. Arrows denote suprabasal nuclei. [Bars = 75 μM (a and b) and 25 μM (c–f).]