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90-226 M1− F1+ expresses PrtF1/SfbI at levels similar to those of JSR4 (M6+ F1+). Equivalent amounts of surface protein extracts were analyzed by Western blotting using anti-SfbI rabbit serum. Lane 1, 90-226 M1− F1+; lane 2, 90-226 M1− pKH3; lane 3, 90-226 M1−; lane 4, JSR4 (M6+ F1+); lane 5, SAM1 (M6+ F1−). Numbers on the right are molecular masses (in kDa).
