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PrtF1/SfbI significantly enhances S. pyogenes strain 90-226 M1− invasion of the HEp-2 epithelial cell line to levels achieved by an M6+ F1+ strain. Invasion assays were performed with 90-226 M1−, 90-226 M1+, 90-226 M1− F1+, and JSR4 (M6+ F1+). Data are presented as percentages of the number of CFU in the inoculum (means ± standard deviations from triplicate experiments). #, P < 0.001, compared to the value obtained with M1− or M1+ invasion, using one-way analysis of variance.
