PrtF1/SfbI promotes phagocytosis resistance in whole blood but not in plasma. The bacterial strains were analyzed for their ability to multiply in nonimmune human blood. Results are from three to five experiments with blood or plasma from two different blood donors. ^, P < 0.01; #, P < 0.001, compared to the value obtained with 90-226 M1− pKH3, using one-way analysis of variance. The multiplication factor (MF) (n-fold increase) was calculated as the mean number of CFU after 3 h divided by the initial mean number of CFU. The isogenic strains analyzed were 90-226 M1+ (M1+), its M-negative mutant (M1−), 90-226 M1− containing the empty expression vector (90-226 M1− pKH3), and 90-226 M1− containing the PrtF1/SfbI vector (M1− F1+).