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Solid-phase Tf-binding assay of whole gonococci. Iron-stressed whole gonococci were applied to nitrocellulose, and Tf binding was examined by probing with peroxidase-conjugated hTf. For both TbpA-expressing (+) and -nonexpressing (−) strains, each lane is labeled according to strain name with the HA epitope insertion number in parentheses. FA19 and FA6815 (TbpA− TbpB−) serve as the wild-type and negative controls, respectively. FA6747 serves as the TbpA− control.
