TABLE 2.
Primers used in this study
| Primer | Sequence (5′-3′)a | Amplification target (HA epitope) |
|---|---|---|
| GC553 | ATTGCCGACAGAACCCA | tbpB |
| GC555 | AAGCACGAGGGTTATGG | tbpB |
| GC556 | CACCGCAAATGGCAATG | tbpB |
| GC561 | CTTCTTTGAGCGGCGGC | tbpB |
| Tfbp-12 | GGCATAAAATATTTAATCGGA | tbpA |
| oVCU136 | ATGAACAATCCATTGGTGAATC | tbpB |
| oVCU137 | GTCCGGGACGTCGTACGGGTAGTCTTTTCGGGCTTCCGG | tbpB (HA1) |
| oVCU138 | CCGTACGACGTCCCGGACTACGCCCAAGGCGGATACGGTTTTGC | tbpB (HA1) |
| oVCU139 | GTCCGGGACGTCGTACGGGTA ACCGTTGGTTTCTACTTCTG | tbpB (HA2) |
| oVCU140 | CCGTACGACGTCCCGGACTACGCCAATTCTAAAATGTACACTTCACCT | tbpB (HA2) |
| oVCU141 | GCCGCCGCTCAAAGAAGAC | tbpB |
| oVCU142 | GCCTATGAATGGAGCAGGC | tonB |
| oVCU143 | GTCCTGCCCTAGCGGGC | tonB |
| oVCU208 | GTCCGGGACGTCGTACGGGTATTGTCGGGAAGGTTTGTCG | tbpB (HA3) |
| oVCU209 | CCGTACGACGTCCCGGACTACGCCCTTCCCGCTTCTGAAGCAG | tbpB (HA3) |
| oVCU210 | GTCCGGGACGTCGTACGGGTACTTGCCGCTGAAGCGGTT | tbpB (HA4) |
| oVCU211 | CCGTACGACGTCCCGGACTACGCCGCGATAGCGACCGACAAAC | tbpB (HA4) |
| oVCU212 | GTCCGGGACGTCGTACGGGTACTCACCCTGCGGGCCGAA | tbpB (HA5) |
| oVCU213 | CCGTACGACGTCCCGGACTACGCCGAATTGGGTTTCCGCTTTTTG | tbpB (HA5) |
| oVCU214 | GTCCGGGACGTCGTACGGGTATTTATCTGCCTGACCGTTCC | tbpB (HA6) |
| oVCU215 | CCGTACGACGTCCCGGACTACGCCGGTAAAAACGGCGGAACAGA | tbpB (HA6) |
| oVCU216 | CGGGCTGTAGGTTGTTTCG | tbpB |
| oVCU217 | GTCCGGGACGTCGTACGGGTACGCGCCTGTTTGGGCTTT | tbpB (HA7) |
| oVCU218 | CCGTACGACGTCCCGGACTACGCCGGCGGCATGCAAACCGCT | tbpB (HA7) |
| oVCU219 | CTCAATCATGGCGTCAATGG | tbpB |
| oVCU220 | GTCCGGGACGTCGTACGGGTACGTACCTTTAAAGCCGTTGC | tbpB (HA8) |
| oVCU221 | CCGTACGACGTCCCGGACTACGCCGCGAAAACCGGTAATGACGG | tbpB (HA8) |
| oVCU224 | GTCCGGGACGTCGTACGGGTATTTCGTTTGTTCATTGCCCG | tbpB (HA9) |
| oVCU225 | CCGTACGACGTCCCGGACTACGCCAATGCAACAGTTGAATCCGG | tbpB (HA9) |
| oVCU226 | CACAAGCTTTTGGCGTTTCGC | tbpB |
| oVCU327 | CCGTACGACGTCCCGGACTACGCCACCGGTGTCGGCAGCAGC | tonB |
| oVCU328 | GTCCGGGACGTCGTACGGGTATCCCTTACCTTCCCCATTACC | tonB |
a
Sequences encoding the HA epitope are underlined.