FIG. 4.

IL-12 restores the BAL cell composition after vaccination and after challenge. (A) Representative scatter plots of BAL cells recovered from the lungs of WT (left) and CD154^−/− mice (right) after vaccination showing size (forward scatter) and granularity (side scatter). (B) Numbers of BAL cell types in WT mice, CD154^−/− mice given saline, and CD154^−/− mice given rIL-12 on day 21 p.v. and (C) on day 15 p.c.. IL-12 was given on days 1, 3, 6, and 10 p.v. Small nongranular lymphocytes (L; open), granulocytes (G; light gray), and large granular macrophages and DCs (LG; dark gray), as determined by flow cytometric analysis, are shown as histogram bars + SEM (error bars) (n = 4 mice/group). Numbers of CD4⁺ and CD11c⁺ cells in BAL are shown as bars + SEM (error bars). Significance values are for CD154^−/− mice given saline (sal.) compared to WT or to CD154^−/− mice given rIL-12.