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Growth and viability of the eca1 mutants are reduced by calcineurin inhibitors. (A). Ten-fold serial dilutions of eca1-11, eca1-26, the insertion mutant AI-5C9, and wild-type (WT) cells were plated on YPD agar or YPD medium supplemented with CsA or FK506 and cultured at 30°C or 35°C for 2 days. (B). Wild-type, eca1, or cna1 cells were plated on YPD medium or YPD medium supplemented with CsA and cultured at 37°C for 2 days before being transferred to 30°C and cultured for 2 more days.
