FIG. 4.

Deletion of ECA1 results in hypersensitivity to multiple stresses. (A) Ten-fold dilutions of wild-type (WT), eca1, or cna1 cells were plated on YPD medium or YPD medium supplemented with 100 mM or 250 mM CaCl₂, 10 mM EGTA, 0.1 μg/ml tunicamycin (Tm), or 1.5 M NaCl and cultured at 30°C or 35°C for 2 days. (B) Wild-type, eca1, or cna1 cells were cultured in RPMI medium (pH 7.0) containing 10-fold serial dilutions of fludioxonil and cultured at 30°C for 2 days. Growth was determined by measuring the optical density at 595 nm. The graph shows the percentage of growth relative to cells cultured in medium containing no fludioxonil.