FIG. 6.
The Eca1 SERCA pump is required for virulence in a murine macrophage model. Cryptococcal yeast cells of the wild type (WT), the eca1 insertion mutant (AI-5C9), and two eca1Δ deletion mutants (eca1-11 and eca1-26) were inoculated into macrophage J774A.1 cultured cells at an MOI of 2:1 (left panel) or into macrophage-free medium (DMEM; right panel). The cryptococcal strains were inoculated at 1 × 106 cells (gray bars), and after 24 h (white bars) of incubation, growth was measured by plating on YPD and counting CFU after culture at 30°C for 2 days. *, P < 0.05.