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Temperature influences the role of C. neoformans Eca1 SERCA pump and calcineurin in virulence in the wax moth G. mellonella. Ten wax moth larvae were injected with 1 × 105 cells of the wild-type or eca1Δ, cna1Δ, or eca1Δ cna1Δ strains and incubated at 30 or 37°C, and survival was monitored daily.
