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Lack of PfRh1 amplification in Senegalese field isolates. Southern blot to determine PfRh1 copy number. Pfdhps is a single-copy gene used as a control. PfRh1 copy number was determined for each sample by comparing the Pfdhps/PfRh1 signal ratio for each Senegalese isolate to that for HB3, which is known to have a PfRh1 copy number of 1.
