Extensive and novel polymorphisms in the repetitive regions of PfRh1, PfRh2a, and PfRh2b. (A) Alignment of PfRh1 repetitive sequence polymorphisms and their allelic designations according to the convention of Lobo et al. (17) (amino acids 2734 to 2741 according to the 3D7 sequence, accession no. AAQ10315). Isolates that contain polymorphisms previously described by Lobo et al. (17): 3D7-LIKE (P18, T14, and T15); FFS-LIKE (P04, P06, T04, T09, and T10); Dd2-LIKE (T22). Isolates that contain novel polymorphisms: NOVEL A (T11, T18, P02, P12, and T13); NOVEL B (P15), and NOVEL C (P09). (B) Alignment of sequence polymorphisms in the repetitive regions of PfRh2a in multiple Senegalese isolates. Polymorphism in the repetitive regions and the “pep” polymorphisms (amino acids 2734 to 2741 according to the FVO sequence, accession no. AAN39446) are indicated in bold. Isolates that contain polymorphisms previously described by Lobo et al. (17), FVO-LIKE (P04, P05, P06, P12, P41, P43, T09, T10, T11, and T13). Isolates that contain novel polymorphisms, NOVEL A (P15 and T15), NOVEL B (P18), and NOVEL C (T14). (C) Alignment of sequence polymorphisms in the repetitive regions of PfRh2b in multiple Senegalese isolates. The repetitive region and “pep” polymorphisms (amino acids 2769 to 2777 according to the 3D7 sequence, accession no. AAN39447) are indicated in bold. Isolates that contain polymorphisms previously described by Lobo et al. (17): 3D7-LIKE (P04,P05,P41,P43,T09,T10,T12,T13,T14,T18) and GVM-LIKE (P06,P15,P18,T22). Isolates that contain novel polymorphisms: NOVEL A (P09), NOVEL B (T11), and NOVEL C (P12). Isolates containing the downstream PfRh2bdel polymorphism are shown in bold.