FIG. 1.

Endogenous SH2-Bβ is present in Listeria actin tails. (A) COS-7 cells were infected with Listeria and stained with anti-SH2-Bβ (green), DAPI (blue), and phalloidin-Texas Red (red). Arrows indicate bacterial actin tails. Boxed regions in the upper right corners are enlarged images of the tails marked with asterisks in the larger images. (B) Macrophages were infected with Listeria and stained with anti-SH2-Bβ (green), DAPI (blue), and phalloidin-Texas Red (red). Arrows indicate bacterial actin tails. (C) Whole-cell lysates of COS-7 cells transfected with (lane 1) or without (lane 2) cDNA encoding SH2-Bβ were subjected to anti-SH2-Bβ Western blotting. The migration of endogenous SH2-Bβ and molecular weight standards is indicated.