FIG. 4.

Cellular localization of proteins of the gp82 family in metacyclic forms of the T. cruzi CL strain. Live or permeabilized parasites were reacted with MAb 3F6 or polyclonal antibodies to J18 or C03 and processed for analysis by flow cytometry or for visualization by fluorescence microscopy. DAPI staining of parasite kinetoplast and nucleus appears in blue. Note the similar expression patterns of MAb 3F6-reactive molecules in live and permeabilized parasites, whereas the molecules recognized by anti-J18 and anti-C03 antibodies were in part localized intracellularly, as indicated by the high fluorescence intensity of permeabilized parasites compared to their live counterparts.