TABLE 2.
Quantitative culture of selected organs from rabbits challenged with B. anthracis
| Time (h) after infection and treatment groupa | Plate count (CFU/0.1 g)b
|
|||||
|---|---|---|---|---|---|---|
| Lung | Spleen | Kidney | Heart | Liver | Brain | |
| 12 | ||||||
| PBS | 423 ± 270 (3/3) | 0 | 0 | 0 | 0 | 0 |
| 21D9 | 537 ± 510 (3/3) | 0 | 0 | 0 | 0 | 0 |
| 24 | ||||||
| PBS | 130 ± 102 (2/3) | 0 | 0 | 0 | 0.67 ± 0.94 (1/3) | 0 |
| 21D9 | 141 ± 76 (3/3) | 0 | 0 | 0 | 0 | 0 |
| 36 | ||||||
| PBS | 6,040 ± 8,038 (3/3) | 220 ± 269 (3/3) | 91 ± 113 (3/3) | 71 ± 99 (2/3) | 4 ± 3 (3/3) | 0.33 ± 0.47 (1/3) |
| 21D9 | 218 ± 77 (3/3) | 1.3 ± 1.9 (1/3) | 0 | 0 | 0 | 0 |
| 48 | ||||||
| PBS | 301 ± 332 (3/3) | 10 ± 14 (1/3) | 8.3 ± 12 (1/3) | 0.33 ± 0.48 (1/3) | 3.3 ± 4 (2/3) | 0 |
| 21D9 | 115 ± 115 (3/3) | 0 | 0 | 0 | 0 | 0 |
| 60 | ||||||
| PBS | 4,944 ± 6,828 (3/3) | 43 ± 61 (1/3) | 2.7 ± 3.8 (1/3) | 0 | 0 | 0 |
| 21D9 | 62 ± 84 (2/3) | 0 | 0 | 0 | 0 | 0 |
| 72 | ||||||
| PBS | —c | — | — | — | — | — |
| 21D9 | 133 ± 124 (3/3) | 0 | 0 | 0 | 0 | 0 |
a
PBS, animals infected with B. anthracis Ames spores alone; 21D9, animals that were passively immunized and infected with anthrax.
b
An aliquot (0.1 g) of the tissue was used for homogenization. The values shown represent the mean CFU (± standard deviation) of B. anthracis from plate counts per 0.1 g of tissue from three rabbits. The values in parentheses indicate the number of rabbits with positive organ culture/number of rabbits per group.
c
—, no sample taken from dead animals.