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MS of PGE2 from C. albicans. C. albicans strain CHN1 was incubated with AA, and putative PGE2 was purified from the supernatants and subjected to LC-MS/MS. (A) Elution profile of PGEx focusing on the m/z range of 350.5 to 351.5. (B) Elution profile of a purified PGE2 standard. Insets in panels A and B demonstrate the MS/MS fragmentation pattern seen at the major peak at 6.4 min. (C) Elution profile of an 8-iso-PGE2 standard (a stereoisomer of PGE2 formed by auto-oxidation of AA).
