FIG. 3.

Effect of COX and LO inhibitors on C. albicans PGE₂ production in whole cells and lysates. C. albicans whole cells (a) and cell-free lysates (b) were treated as described in the legend to Fig. 2. Inhibitors dissolved in dimethyl sulfoxide were added at the time of AA addition. Both whole-cell supernatants and cell-free lysates (with protein concentrations normalized) were assayed for PGE₂ production using a monoclonal PGE₂ EIA kit (Cayman Chemicals, Ann Arbor, MI). Experiments were performed in duplicate, and results are the averages for two independent experiments which were repeated three times with similar results. Initials indicate which drugs exhibit statistically significant inhibition at each concentration. A, aspirin; I or Indo, indomethacin; N, NDGA; R or Res, resveratrol; C or Cay, CAY10404. *, P < 0.05, no inhibitor versus inhibitor.