FIG. 5.

Role of C. albicans FET3 in PGE₂ production. (b) C. albicans strain SC5314 and fet3/fet3 mutant whole cells (a) or cell-free lysates (b) were treated as described in the legend to Fig. 2 (9). Both whole-cell supernatants and lysates (with protein concentrations normalized) were assayed for PGE₂ production using a monoclonal PGE₂ EIA kit (Cayman Chemicals, Ann Arbor, MI). The amount of detectable oxidized AA observed in control samples containing AA alone for each time point was subtracted from the experimental samples. The percentage of control PGE₂ production was determined by the formula [(PGE₂ produced by mutant strain)/(PGE₂ produced by parental strain)] × 100). Experiments were performed in duplicate, and results represent the average of three independent experiments. *, P < 0.05, SC5314 versus fet3/fet3 mutant.