FIG. 3.
Involvement of HSPGs in CNF1 and CNFY uptake into HeLa cells. HeLa cells were preincubated with sodium chlorate (80 mM) for 48 h at 37°C. Cells were then stained for surface HSPGs and analyzed by fluorescence microscopy (A), or they were treated with GST-CNF1 (1 nM) for 1 h or with GST-CNFY (1 nM) for 2.5 h at 37°C in the presence of sodium chlorate. Following cell lysis, a Rho shift assay was performed to investigate the influence of NaClO4 on intoxication (B). Note that a lot of cells were lost during sodium chlorate treatment.