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Role of lipid rafts in endocytosis of CNF1 and CNFY. HeLa cells were pretreated with nystatin (25 μM) for 1 h at 37°C. Cells were then intoxicated with bacterial supernatant containing VacA for 2 h, GST-CNF1 (1 nM) for 1 h, or GST-CNFY (1 nM) for 2.5 h at 37°C in the presence of nystatin. To study the effect of nystatin on CNF intoxication, a Rho shift assay was performed (A). For VacA intoxication, cells were analyzed morphologically by phase-contrast microcopy (B). Nys, nystatin.
