TABLE 3.
Effect of ryhB expression on paraquat sensitivity of S. dysenteriae
| Strain | Plasmid | Growth (optical density at 650 nm)a
|
|
|---|---|---|---|
| Without paraquat | With paraquat | ||
| ND100 (wild type) | Vector | 1.16 ± 0.03 | 0.88 ± 0.07 |
| ND100 (wild type) | pryhB | 0.87 ± 0.03 | 0.24 ± 0.03b |
| ND100fur | Vector | 0.62 ± 0.03 | 0.37 ± 0.02b |
| ND100fur/ryhB | Vector | 1.18 ± 0.02 | 1.02 ± 0.07 |
| ND100ryhB | Vector | 1.12 ± 0.03 | 1.06 ± 0.07 |
a
An endpoint growth analysis of S. dysenteriae wild-type strain ND100, S. dysenteriae fur mutant ND100fur, S. dysenteriae fur ryhB double mutant ND100fur/ryhB, and S. dysenteriae ryhB mutant ND100ryhB containing the vector control or pryhB in the presence or in the absence of 1 μM paraquat was performed. The data are the averages of three independent experiments. All strains were cultured in the presence of 200 μM IPTG to induce expression of ryhB from pryhB.
b
Significantly different than the growth of the wild-type strain in the presence of paraquat (P ≤ 0.004).