TABLE 2.
Comparison of technical parameters among Nanogen GNEN, Genaco ResPlex II, and monoplex TaqMan methods for detection of RNA virus respiratory pathogens
| Parameter | GNEN | ResPlex II | TaqMan |
|---|---|---|---|
| Amplification platform | Multiplex RT-PCR | Multiplex RT-PCR | Monoplex RT-PCR |
| Detection format | Nanochip (solid chip) | Luminex (liquid chip) | TaqMan (real time) |
| Pathogens covered | Flu-A, Flu-B, PIV-1, PIV-2, PIV-3, RSV | Flu-A, Flu-B, PIV-1, PIV-2, PIV-3, PIV-4, RSV-A, RSV-B, hMPV, RhV, EnV, SARS CoV | Flu-A, Flu-B, PIV-1, PIV-2, PIV-3, RSV, hMPV |
| No. of reactions needed | 1 | 1 | 7 |
| Hands-on time (min)a | 70 | 55 | 135 |
| Detection throughput (no. of tests/run) | 80 | 96 | 13.5 |
| Test turnaround time (h)b | 9 | 6 | 4 |
a
Hands-on and turnaround times include time needed for specimen processing and data analysis.
b
Times are based on a full run, including specimen processing, target amplification, and product identification.