TABLE 1.

Primers and probes used for real-time PCR detection

Virus(es)	Target gene	Forward primer(s) (5′-3′)	Reverse primer(s) (5′-3′)	Probe(s)a	Reference
RSV A	Nucleocapsid	AGA TCA ACT TCT GTC ATC CAG CAA	TTC TGC ACA TCA TAA TTA GGA GTA TCA AT	FAM-CAC CAT CCA ACG GAG CAC AGG AGA T-TAMRA	8
RSV B	Nucleocapsid	AAG ATG CAA ATC ATA AAT TCA CAG GA	TGA TAT CCA GCA TCT TTA AGT ATC TTT ATA GTG	FAM-TTC CCT TCC TAA CCT GGA CAT AGC ATA TAA CAT ACC T-TAMRA	8
IV A	Matrix	AAG ACC AAT CCT GTC ACC TCT GA	CAA AGC GTC TAC GCT GCA GTC C	FAM-TTT GTG TTC ACG CTC ACC GT-TAMRA	10
IV B	Hemagglutinin	AAA TAC GGT GGA TTA AAC AAA AGC AA	CCA GCA ATA GCT CCG AAG AAA	FAM-CAC CCA TAT TGG GCA ATT TCC TAT GGC-TAMRA	7
PIV 1	Hemagglutinin-neuraminidase	TGA TTT AAA CCC GGT AAT TTC TCA T	CCT TGT TCC TGC AGC TAT TAC AGA	FAM-ACG ACA ACA GGA AAT C-MGB
PIV 2	Hemagglutinin-neuraminidase	AGG ACT ATG AAA ACC ATT TAC CTA AGT GA	AAG CAA GTC TCA GTT CAG CTA GAT CA	FAM-ATC AAT CGC AAA AGC TGT TCA GTC ACT GCT ATA C-TAMRA
PIV 3	Hemagglutinin-neuraminidase	TGA TGA AAG ATC AGA TTA TGC ATA TC	CCG GGA CAC CCA GTT GTG	FAM-TGG ACC AGG GAT ATA CTA CAA AGG CAA AAT AAT ATT TCT C-TAMRA
PIV 4	Nucleocapsid	CAA AYG ATC CAC AGC AAA GAT TC	ATG TGG CCT GTA AGG AAA GCA	FAM-GTA TCA TCA TCT GCC AAA TCG GCA ATT AAA CA-TAMRA
AVs	Hexon	TTT GAG GTG GAY CCM ATG GA	AGA ASG GSG TRC GCA GGT A	FAM-ACC ACG TCG AAA ACT TCG AA-MGB
		TTT GAG GTY GAY CCC ATG GA	AGA ASG GTG TRC GCA GAT A	FAM-ACC ACG TCG AAA ACT TCA AA-MGB
				FAM-ACA CCG CGG CGT CA-MGB

^aFAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine; MGB, minor groove binding.