TABLE 2.

Intra-assay variation of the hBoV real-time PCR assay for different concentrations of the plasmid pBoV1

No. of copies/ reaction	CT
	Value for replicate:			Mean	SD	CV (%)a
	1	2	3
3.8 E + 08	12.82	12.37	12.19	12.46	0.32	2.60
3.8 E + 07	15.94	15.90	15.93	15.92	0.02	0.13
3.8 E + 06	19.19	19.17	19.08	19.15	0.06	0.31
3.8 E + 05	22.60	22.62	22.62	22.61	0.01	0.05
3.8 E + 04	26.02	25.97	26.03	26.01	0.03	0.12
3.8 E + 03	29.41	29.47	29.20	29.36	0.14	0.48

^aCV, coefficient of variation.