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. 1998 Mar 3;95(5):2313–2318. doi: 10.1073/pnas.95.5.2313

Figure 3.

Figure 3

Binding of radiolabeled CRE to hippocampal neurons (in situ Southwestern assay). (A–C) binding of CRE concatamer to hippocampal neurons at 48 hr in culture (darkfield, 150×). Grains are very dense in nucleus (arrowhead) similar to CREB immunoreactivity but are also detected within the dendrite and dendritic growth cones (arrow). There was no clear binding to axons. (D) Phase contrast view of (C). (E) Unligated CRE exhibited no binding to the neurons. (F) No signal was observed with a ligated inverted CRE palindrome sequence (magnification, ×50).