TABLE 2.
Summary of discordant HPV results
| HR-HPV resulta | No. of discordant results
|
Total no. of specimens | |||||
|---|---|---|---|---|---|---|---|
| LA
|
AMP
|
HC2
|
|||||
| Positive | Negative | Positive | Negative | Positive | Negative | ||
| HC2+/AMP− | 1b | 51c | 52 | ||||
| HC2−/AMP+ | 195d | 14e | 209 | ||||
| Total | 261 | ||||||
| HC2+/LA− | 11f | 51c | 62 | ||||
| HC2−/LA+ | 195d | 11g | 206 | ||||
| Total | 268 | ||||||
| AMP+/LA− | 11f | 14e | 25 | ||||
| AMP−/LA+ | 1b | 11g | 12 | ||||
| Total | 37 | ||||||
An LA+ result refers to the detection of a HR-HPV genotype; likewise, an LA− result refers to the absence of an HR-HPV genotype.
Specimen shown to have HPV genotype 45 (by LA).
Specimens possibly representing HC2 cross-reactive genotypes. A total of 32 had at least one of the types 53, 66, 67, 70, 71, 73, or CP6108, and 13 had one of the types 6, 11, 26, 40, 42, 83, or 84, totaling 45 of 51 (88.2%) with known cross-reactive types (type 53 [n = 15], type 42 [n = 9], type 66 [n = 6], type 73 [n = 6], type CP6108 [n = 6], and type 6 [n = 5]). The other six specimens were nondetectable types (n = 4) or HPV-54 and HPV-61.
Due to increased sensitivity of the two PCR-based assays.
Nine specimens had nondetectable HPV types, the other five had exclusively LR types: 55 and 62; 6, 55, 72, and 84; 61 and 62; CP6108; or 54.
Possibly due to a combination of lower sensitivity of the LA and HC2 cross-reactive types, since seven contained at least one of the types (53, 66, 67, 70, 71, 73, or CP6108) and a further two contained one of the types (6, 11, 26, 40, 42, 83, or 84), totaling 9 of 11 (81.8%) with known cross-reactive types (66 [n = 6] and CP6108 [n = 2]).
Specimens with HR-HPV types 16 (n = 1), 18 (n = 1), 45 (n = 2), 52 (n = 2), 58 (n = 2), 59 (n = 2), and 68 (n = 1).