TABLE 2.
Characteristics of C. jejuni strains and results of the PCR-RFLP and hybridization analyses for the LOS biosynthesis locus
| Straina | Serotype(s)b | LOS class | Ganglioside epitopec
|
Gened
|
|||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Any | GM1 | GQ1b | 1 | 2 | 3 | 4 | 5 | 14 | 15 | 6 | 7ab | 7c | 8 | 9 | 5/10 | 10 | 11 | 16 | 17 | 12 | 13 | ||||||||
| GB2 | UT | A | + | + | − | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| GB3 | 19 | A | + | + | − | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| GB11 | 2 | A | + | + | − | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| GB16 | 13, 66 | A | + | − | + | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| GB18 | 19 | A | + | + | − | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| GB19 | 4, 50 | A | + | − | + | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| GB21 | 13, 65 | A | + | + | − | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| GB22 | 13, 64 | A | + | + | − | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| GB23 | 4, 13, 43 | A | + | − | − | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| GB26/27 | 1, 44 | A | + | − | − | + | + | + | + | + | − | − | − | + | − | + | + | − | + | + | − | − | + | + | |||||
| GB28 | 19, 38 | A | + | + | − | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| OH4382 | 19 | A | + | − | + | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| GB5 | 4, 64 | B | − | − | − | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| MF6 | 4, 64 | B | + | − | + | + | + | + | + | + | − | − | − | + | − | + | + | − | + | + | − | − | + | + | |||||
| MF7 | 35 | B | + | − | + | + | + | + | + | + | − | − | − | + | − | + | + | − | + | + | − | − | + | + | |||||
| MF8 | 23, 36 | B | + | − | + | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| GB17 | 4, 13, 64 | B | + | − | + | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| MF20 | 2 | B | + | − | + | + | + | + | + | + | − | − | − | + | − | + | + | − | + | + | − | − | + | + | |||||
| GB25 | 2 | B | + | − | + | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| GB1 | 1 | C | − | − | − | + | + | + | + | + | + | + | + | − | + | + | + | + | − | − | + | + | + | + | |||||
| GB13/14 | 2 | C | + | + | − | + | + | + | + | + | + | + | + | − | + | + | + | + | − | − | + | + | + | + | |||||
| GB15 | 5, 34 | F | − | − | − | + | + | + | − | − | − | − | − | − | − | − | − | − | − | − | − | − | + | + | |||||
| GB24 | 31 | K | − | − | − | + | + | + | − | − | − | − | − | − | − | − | − | − | − | − | − | − | + | + | |||||
| GB4 | 37 | P | − | − | − | + | + | − | − | + | − | − | − | − | − | − | − | − | − | − | − | − | + | + | |||||
| HS:4 | 4 | A | + | + | − | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| HS:10 | 10 | A | + | − | + | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| HS:19 | 19 | A | + | + | − | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| HS:23 | 23 | B | − | − | − | + | + | + | + | + | − | − | − | + | − | + | + | − | + | + | − | − | + | + | |||||
| HS:35 | 35 | B | − | − | − | + | + | + | + | + | − | − | − | + | − | + | + | − | + | + | − | − | + | + | |||||
| HS:36 | 36 | B | + | − | − | + | + | + | + | + | − | − | + | + | − | + | + | − | + | + | − | − | + | + | |||||
| HS:1 | 1 | C | + | − | − | + | + | + | + | + | + | + | + | − | + | + | + | + | − | − | + | + | + | + | |||||
| HS:2 | 2 | C | + | − | − | + | + | + | + | + | + | + | + | − | + | + | + | + | − | − | + | + | + | + | |||||
| 11168 | 2 | C | + | + | − | + | + | + | + | + | + | + | + | − | + | + | + | + | − | − | + | + | + | + | |||||
| HS:64 | 64 | D | − | − | − | + | + | + | − | − | − | − | + | − | − | + | + | − | − | − | − | − | + | + | |||||
| HS:3 | 3 | H | − | − | − | + | + | + | − | − | − | − | + | − | − | + | + | − | − | − | − | − | + | + | |||||
| E98-652 | 2 | B | + | + | + | + | + | − | − | + | − | − | − | + | − | + | + | − | + | + | − | − | + | + | |||||
| E98-1033 | 10 | B | + | + | − | + | + | + | + | + | − | − | + | + | − | + | + | − | + | − | − | − | + | + | |||||
| E98-682 | 1 | C | + | + | − | + | + | + | + | + | + | + | + | − | + | + | + | + | − | − | + | + | + | + | |||||
| E98-1087 | 1, 10, 44 | C | + | + | − | + | + | + | + | + | + | + | − | − | + | − | + | − | − | − | + | + | + | + | |||||
| E98-706 | 31, 63 | D | − | − | − | + | + | + | − | − | − | − | − | + | − | − | − | − | − | − | − | − | + | + | |||||
| E98-623 | 6, 57 | E | − | − | − | + | + | − | − | + | − | − | − | + | − | + | + | − | + | − | − | − | + | + | |||||
| E98-624 | 22, 57 | E | − | − | − | + | + | − | − | + | − | − | − | − | − | − | − | − | − | − | − | − | + | + | |||||
| No. of RFLP typese | 17 | 17 | 1-2 | 1-1 | 1 | 1 | 1 | 3-10 | 8 | 1 | 2-9 | 1-8 | 1 | 7 | 1 | 1 | 2 | 1-6 | 9 | ||||||||||
Neuropathy-associated strains: GBxx and OH4382, GBS-associated isolates; MFxx, MFS-associated isolates. Enteritis-associated strains: Exx, isolates from enteritis patients; HS:xx, Penner (HS) type strains; 11168, NCTC 11168 genome strain.
Penner (HS) serotypes; UT, untypeable.
+, present, −, absent. The presence of ganglioside epitopes was determined previously by immunological methods or, when available, mass spectrometry (2, 3, 6).
Gene numbers are open reading frame (ORF) numbers (as shown in Table 1). +, detected by PCR/hybridization analysis; −, not detected by PCR/hybridization analysis.
Data represent the number of RFLP types detected per gene. In cases in which the PCR analysis was performed with two primer sets, two numbers are displayed; the first is the number of RFLP types based on NCTC 11168 primer set PCR, and the second is the number of RFLP types based on HS:19 (OH4384) primer set PCR.