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. 2007 Apr 4;45(6):2090–2091. doi: 10.1128/JCM.02435-06

FIG. 2.

FIG. 2.

PCR gel with DNA extracted from centrifuged CSF samples over a 2-month period, amplified with a 161-bp primer set for Acanthamoeba. An arrow indicates 161 bp. Lane 1, molecular size marker; lane 2, CSF sample (June 2003) showing a weak DNA band; lane 3, CSF sample from ∼10 days later also showing a weak DNA band, along with multiple bands, probably from leukocytes present in the sample; lane 4, CSF sample from ∼3 weeks later; lane 5, CSF sample from ∼4 weeks later (August) at the time of the patient's death; lane 6, Acanthamoeba polyphaga, positive control; lane 7, negative control, pure water (Sigma).