TABLE 1.

CPS type-specific primers and prediction of PCR products by computer simulation

Primer name	Sequence (5′ to 3′)	Gene target(s)	Amplicon size(s) (bp)	GenBank accession no. of targeted operon
Ia-F	GGTCAGACTGGATTAATGGTATGC	cps1aH		AB028896
Ia-R	GTAGAAATAGCCTATATACGTTGAATGC	cps1aH	521 and 1,826
Ib-F	TAAACGAGAATGGAATATCACAAACC	cps1bJ		AB050723
Ib-R	GAATTAACTTCAATCCCTAAACAATATCG	cpsIbK	770
II-F	GCTTCAGTAAGTATTGTAAGACGATAG	cps2K		AY375362
II-R	TTCTCTAGGAAATCAAATAATTCTATAGGG	cps2K	397
III-Fa	TCCGTACTACAACAGACTCATCC	cps1a/2/3I		AF163833
III-Ra	AGTAACCGTCCATACATTCTATAAGC	cps1a/2/3J	1,826
IV-F	GGTGGTAATCCTAAGAGTGAACTGT	cps4N		AF355776
IV-R	CCTCCCCAATTTCGTCCATAATGGT	cps4N	578
V-F	GAGGCCAATCAGTTGCACGTAA	cps5O		AF349539
V-R	AACCTTCTCCTTCACACTAATCCT	cps5O	701
VI-F	GGACTTGAGATGGCAGAAGGTGAA	cps6I		AF337958
VI-R	CTGTCGGACTATCCTGATGAATCTC	cps6I	487
VII-F	CCTGGAGAGAACAATGTCCAGAT	cps7M		AY376403
VII-R	GCTGGTCGTGATTTCTACACA	cps7M	371
VIII-F	AGGTCAACCACTATATAGCGA	cps8J		AY375363
VIII-R	TCTTCAAATTCCGCTGACTT	cps8J	282
dltS-F	AGGAATACCAGGCGATGAACCGAT	dltS		AL766853
dltS-R	TGCTCTAATTCTCCCCTTATGGC	dltS	952

^aPrimers III-F and III-R never yielded the expected amplicon with DNA templates extracted from all GBS serotype II strains tested in this study.